Protein Name & Synonyms:
ELISA (dilution: 1/500, 1/2000), Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml
This product detects human Complement 3c, a polypeptide fragment which is present in C3. C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg.Defects in C3 can result in susceptibility to pyogenic infection.
Store at +4 °C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Phosphate buffered saline
12 months from the date of shipment when stored properly.
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.