Sheep Anti-Human LOX-1

Sheep Anti-Human LOX-1

$542.00
DS-PB-01251
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Antigen Information

Protein Name & Synonyms:
LOX-1
Target Species:
  • Human

Antibody Specifications

Concentration:
IgG concentration 0.1 mg/ml
Preparation:
Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Specificity
This antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting.

Specificity

This product recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.

This product will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels.

Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen
Recombinant human extracellular domain LOX-1 protein from bacteria.
Preservative:
0.09%Sodium Azide
1%Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.