Sheep Anti-Leptin

Sheep Anti-Leptin

$420.00
DS-PB-03025
+

Antigen Information

Protein Name & Synonyms:
Leptin
Target Species:
  • Human
  • Mouse
  • Rat

Antibody Specifications

Applications:
ELISA (dilution: 1/10,000, 1/50,000)
Concentration:
IgG concentration 5.0mg/ml
Preparation:
Antisera to leptin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.
Species Reactivity:
Human, Mouse, Rat

Specificity

This product recognizes leptin, a 16kDa secreted protein hormone encoded by the OB gene, which plays a key role in the regulation of metabolism, body weight and reproduction and is expressed primarily by white adipocytes, and to a lesser extent by stomach endothelium and the placenta.

Regulation of food intake and energy expenditure by leptin is thought to predominantly occur in the hypothalamus of the brain, where leptin binds with high affinity to neuronal leptin receptors (OB-R), inhibiting the action of the feeding stimulants neuropeptide Y (NPY) and agouti-related peptide (AgRP), or promoting the effects of the appetite regulator α-melanocyte-stimulating hormone (α-MSH). Leptin also acts as a direct stimulator of fatty acid oxidation in the mitochondria of liver and skeletal muscle cells, and influences glucose and fat metabolism.

The expression of leptin in relation to metabolic status is influenced by hormonal and metabolite control: stimulators of leptin include insulin and cortisol, whilst cAMP, thiazolidinediones and beta-adrenergic receptor agonists, reduce leptin expression.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to leptin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G.

Immunogen:
HMW conjugated leptin amino acids 57-70.
Preservative:
0.09%Sodium Azide (NaN3)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.