MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK.
Protein Name & Synonyms:
Single-strand selective monofunctional uracil DNA glycosylase, SMUG1, FDG, UNG3, HMUDG.
Greater than 95% as determined by SDS-PAGE.
Sterile Filtered colorless solution.
The SMUG1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 1mM DTT.
SMUG1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 293 amino acids (1-270) and having a molecular mass of 32.3kDa.SMUG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.