Rabbit Anti-Human TBPL2 (N-term)

Rabbit Anti-Human TBPL2 (N-term) Antibody, 400 ul
CODE:  102-11423

Product Specifications

Size:
400 ┬Ál

Protein Information

Accession Number:
  • Q6SJ96
Gene ID:
  • 387332
Gene Symbols:
  • TBPL2
Protein Name & Synonyms:
TBPL2 (N-term)
Target Species:
  • Human

Antibody Specifications

Concentration (lot specific):
0.380 mg/ml
Preparation:
This TBPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TBPL2.
Species Reactivity:
Human
Clonality:
Polyclonal

Introduction

TBPL2 is a transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process (By similarity).

Specificity

Other Names
TBPL2; TBP2; TRF3; TATA box-binding protein-like protein 2; TATA box-binding protein-related factor 3
NCBI Accession #
NP_950248.1
Other Accession #
NP_950248.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
41524
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
TBPL2 Antibody (N-term) (Cat# 102-11423) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).
TBPL2 Antibody (N-term) (Cat# 102-11423)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with TBPL2 Antibody (N-term)(Cat# 102-11423).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBPL2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBPL2 immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.