Protein Name
Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84)
Synonyms
Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, Heat Shock Protein 90 Alpha Family Class B Member 1, Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1, Heat Shock Protein 90kDa Alpha Family Class B Member 1, Heat Shock 90kD Protein 1 Beta, HSP90B, HSPC2, HSPCB, Heat Shock 90kDa Protein 1 Beta, Heat Shock Protein 90 KDa, HSP90-Beta, D6S182
Species
Human
Source
Expression System
Human Embryonic Kidney 293 (HEK293) Cells
Gene Symbol
HSP90AB1 HSP90B HSPC2 HSPCB
Expressed Region
Pro2-Asp724
Conjugation/Tag
N-terminal histidine tag
Molecular Mass (kDa)
Recombinant protein has a calculated molecular weight of about 83 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
Preparation
Preparation
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Purity
Unpurified cell lysate
Purity determined by
Not applicable
Specifications
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Concentration
Determined by Bio-Rad protein assay reagent
Endotoxin Level
Not determined
Recommended Applications
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.
Stability & Storage
Shipping
Including one vial of HEK293 cell lysate transfected with empty expression vector.
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.