Discovery Proteomics Services

Efficiently identify key proteins of interest and post-translational modifications (PTMs) when you maximize your protein expression discovery efforts with RayBiotech’s Discovery Proteomics Services.

We use our semi-quantitative, high-density arrays for this service, and work with you to determine whether our label-based or sandwich-based technologies will best achieve your project goals.

Regardless of which technology you choose, we’ll maximize the number of proteins that are simultaneously assessed. We can use any of our antibody array products to quickly screen for biomarkers, PTMs, and other targets across 8000 human, 2808 mouse, 1500 rat, 50 porcine, 30 bovine, or 500 rabbit proteins, or we can create a custom array to meet your project needs.

Request a Quote Sample Shipment Instructions

When to Choose Our Discovery Proteomics Services

We recommend choosing this service when you need to maximize the number of proteins that are analyzed, sample volume is not limiting, and semi-quantitative results are sufficient.

Why You Should Choose Our Discovery Proteomics Services

For Our Patented Array Technologies and Proven Workflows

This service is delivered using off-the-shelf or custom RayBiotech Label-Based Arrays (L-Series) or Glass Slide Antibody Arrays (G-Series).

Label-Based Arrays maximize the number of targets and PTMs like acetylation, palmitoylation, nitrosylation, oxidation, glycosylation, or phosphorylation you can assess. This approach relies on direct labeling of sample proteins with biotin, enabling detection through binding with labeled streptavidin and avoiding the need to develop validated antibody pairs. Learn more about the PTM analyses available.

8000 Human Proteins

6000 Human Proteins

4000 Human Proteins

2000 Human Proteins

1000 Human Proteins

507 Human Proteins

500 Human Proteins

Number of Targets

500

Sample Types

Serum, plasma, cell culture media

Sample Volume Needed

20 ul (120 ul for cell culture media, 20 ug for lysates)

Kit Used

Human L3 Array, Glass SlideHuman L4 Array, Glass SlideHuman L5 Array, Glass SlideHuman L6 Array, Glass SlideHuman L7 Array, Glass SlideHuman L8 Array, Glass SlideHuman L9 Array, Glass SlideHuman L10 Array, Glass SlideHuman L11 Array, Glass SlideHuman L12 Array, Glass SlideHuman L13 Array, Glass SlideHuman L14 Array, Glass SlideHuman L15 Array, Glass SlideHuman L16 Array, Glass Slide

493 Human Proteins

2808 Mouse Proteins

1308 Mouse Proteins

500 Mouse Proteins

308 Mouse Proteins

1500 Rat Proteins

500 Rat Proteins

500 Rabbit Proteins

Glass Slide Antibody Arrays use the familiar sandwich detection format and our validated antibody pairs.

1200 Human Proteins

Human Kiloplex

640 Human Proteins

440 Human Proteins

200 Human Proteins

640 Mouse Proteins

400 Mouse Proteins

200 Mouse Proteins

282 Rat Proteins

67 Rat Proteins

50 Porcine Proteins

30 Bovine Proteins

30 Rabbit Proteins

Learn more about how array technology works

For Our Years of Experience

Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding proteomics services to the research community.

What you can expect

RayBiotech’s Discovery Proteomics Service process is streamlined and straightforward:

You:

Finalize your service agreement.
Prepare your samples for shipment—you can find instructions here.
Fill out a Service Form and include it with your shipment.
Ship us your samples (note that there is a 4 sample minimum for this service).

RayBiotech

Once we receive your samples, we’ll send you an email.
We’ll conduct your studies, keeping you informed about any issues or delays.
We’ll send back a detailed data analysis in Excel and PDF formats.

Ready for RayBiotech to start working on your Discovery Proteomics Service?

Request a Quote

Frequently Asked Questions

Still have questions?

What types of samples are compatible with the arrays?

Most of our arrays are compatible with any biological fluid. This includes cell culture media, cell lysates, tissue lysates, and all clarified body fluids (serum, plasma, urine, cerebrospinal fluid, BAL, saliva, tears, etc.)

However,the label-based arrays (L-Series), have limited compatibility with lysates and non-serum/plasma body fluids. Our phosphorylation arrays, which detect membrane-bound receptors, are only validated for use with cell and tissue lysates.

In general, any cell-free extract that contains soluble, non-denatured proteins is likely to work with our arrays.

What is the difference between the membrane-based and glass slide-based arrays?

There are a few differences between the glass-slide and membrane-based arrays. The main difference concernsdetection methodology. Our glass-slide based arrays use fluorescence and need a compatible laser scanner. However, our membrane-based arrays use chemiluminescence that is detectable with most western blot imaging systems.

The sample size needed for the glass-slide based is smaller than the membrane-based. Glass-slides require ~70 ul – 100 ul, while the membranes require at least 1 ml, after dilution.

Lastly, the price for the glass-slide based arrays are generally cheaper.

What are the positive and negative controls on the arrays?

The positive control spots are standardized amounts of biotinylated IgG. They are used for signal normalization, monitoring of the detection step, and to help orient the array image.

The negative control spots are printed with buffer only, and are not expected to give signals. Negative control spots are used for background subtraction.

The areas on the array map labeled as “blank” are empty (there is nothing printed there). Both the negative control and blank spots should give similar intensity values. Either one may be used to represent the background.

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