Antibody Production Services

Custom Antibody Production Services

RayBiotech offers a complete custom antibody production service covering every step of monoclonal and polyclonal development. Our capabilities include peptide antigen design (including phospho peptides), synthesis and carrier protein conjugation, immunization, titer analysis, serum collection or hybridoma fusion, and antibody purification. Many customization options are available, such as antibody conjugation, lyophilization, custom vialing & aliquoting, and antibody validation by Western blot, ELISA, or immunoprecipitation.

Custom Antibody Production Services

Service Items

Polyclonal Antibody Production (pAb)

Monoclonal Antibody Production (mAb)

Description

Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, purification

Recognizing one antigenic binding site of the antigen, immunization, fusion, screening, subclone, characterization, purification

Size

150-900 kDa

150 kDa

Animal use

Yes

Period

About 2 months

4-6 months

Specificity

Low

High

Affinity

High

Variable

Binding site

Multiple

Single

Amount

Limited

Reproducible

Service items	Lead Time	Description	Deliverables
Anti-regular peptide polyclonal antibody (customer-provided peptide)	5 weeks	Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-regular peptide polyclonal antibody (we make the peptide)	8 weeks	Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-phospho-peptide polyclonal antibody	8-10 weeks	Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-protein polyclonal antibody	5-7 weeks	Customer provides 5 mg protein antigen Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody

Service items

Lead Time

Description

Deliverables

Anti-regular peptide polyclonal antibody (customer-provided peptide)

5 weeks

Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-regular peptide polyclonal antibody (we make the peptide)

8 weeks

Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-phospho-peptide polyclonal antibody

8-10 weeks

Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-protein polyclonal antibody

5-7 weeks

Customer provides 5 mg protein antigen
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Service Items	Time	Description
Anti-regular peptide antibody (customer provide antigen)	4-5 months	Customer provides peptide (~4mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-regular peptide antibody (RayBiotech provide antigen)	4-5 months	Design, synthesize and conjugate peptide (15-20mer,5-10mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-phospho-peptide antibody	5-6 months	Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Antibody purification by affinity column (1-5mg) may be obtained upon request
Anti-protein antibody	5 months	4mg of purified Protein from customer Immunize up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Antibody purification by affinity column (1-5mg) may be obtained upon request

Service Items

Time

Description

Anti-regular peptide antibody (customer provide antigen)

4-5 months

Customer provides peptide (~4mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

Anti-regular peptide antibody (RayBiotech provide antigen)

4-5 months

Design, synthesize and conjugate peptide (15-20mer,5-10mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

Anti-phospho-peptide antibody

5-6 months

Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Antibody purification by affinity column (1-5mg) may be obtained upon request

Anti-protein antibody

5 months

4mg of purified Protein from customer
Immunize up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Antibody purification by affinity column (1-5mg) may be obtained upon request

Service Items	Polyclonal Antibody Production (pAb)	Monoclonal Antibody Production (mAb)
Description	Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, purification	Recognizing one antigenic binding site of the antigen, immunization, fusion, screening, subclone, characterization, purification
Size	150-900 kDa	150 kDa
Animal use	Yes	Yes
Period	About 2 months	4-6 months
Specificity	Low	High
Affinity	High	Variable
Binding site	Multiple	Single
Amount	Limited	Reproducible

Antibody purification and conjugation takes around 2-3 days and includes conjugation of polyclonal or monoclonal antibodies to enzymes, fluorochromes, DNA, or affinity ligands. Solid surface conjugation is also available.

Service Items

Polyclonal Antibody Production (pAb)

Monoclonal Antibody Production (mAb)

Description

Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, purification

Recognizing one antigenic binding site of the antigen, immunization, fusion, screening, subclone, characterization, purification

Size

150-900 kDa

150 kDa

Animal use

Yes

Period

About 2 months

4-6 months

Specificity

Low

High

Affinity

High

Variable

Binding site

Multiple

Single

Amount

Limited

Reproducible

Service items	Lead Time	Description	Deliverables
Anti-regular peptide polyclonal antibody (customer-provided peptide)	5 weeks	Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-regular peptide polyclonal antibody (we make the peptide)	8 weeks	Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-phospho-peptide polyclonal antibody	8-10 weeks	Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg) Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody
Anti-protein polyclonal antibody	5-7 weeks	Customer provides 5 mg protein antigen Immunization of two rabbits (other species available) Perform test bleeds, titer tests Purification of serum by protein A/G column Antigen affinity purification may be obtained upon request	10 mg purified antibody

Service items

Lead Time

Description

Deliverables

Anti-regular peptide polyclonal antibody (customer-provided peptide)

5 weeks

Customer provides peptide: 1 mg non-conjugated; 2 mg KLH-conjugated (90% purity)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-regular peptide polyclonal antibody (we make the peptide)

8 weeks

Design, synthesis and conjugation of peptide (15-20-mer, 5-10 mg)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-phospho-peptide polyclonal antibody

8-10 weeks

Design, synthesis, and conjugation of phosphorylated peptide (15-20-mer, 5-10 mg)
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Anti-protein polyclonal antibody

5-7 weeks

Customer provides 5 mg protein antigen
Immunization of two rabbits (other species available)
Perform test bleeds, titer tests
Purification of serum by protein A/G column
Antigen affinity purification may be obtained upon request

10 mg purified antibody

Service Items	Time	Description
Anti-regular peptide antibody (customer provide antigen)	4-5 months	Customer provides peptide (~4mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-regular peptide antibody (RayBiotech provide antigen)	4-5 months	Design, synthesize and conjugate peptide (15-20mer,5-10mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
Anti-phospho-peptide antibody	5-6 months	Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg) Immunization of up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Antibody purification by affinity column (1-5mg) may be obtained upon request
Anti-protein antibody	5 months	4mg of purified Protein from customer Immunize up to five mice, fusion, screening, subclone and initial characterization With up to three monoclones and their supernatant for confirming detection Generated antibody recognizes immunogen in ELISA assay Antibody purification by affinity column (1-5mg) may be obtained upon request

Service Items

Time

Description

Anti-regular peptide antibody (customer provide antigen)

4-5 months

Customer provides peptide (~4mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

Anti-regular peptide antibody (RayBiotech provide antigen)

4-5 months

Design, synthesize and conjugate peptide (15-20mer,5-10mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid

Anti-phospho-peptide antibody

5-6 months

Design, synthesize, specific site phophorylation and conjugate peptide (11-15mer, 5 mg)
Immunization of up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Antibody purification by affinity column (1-5mg) may be obtained upon request

Anti-protein antibody

5 months

4mg of purified Protein from customer
Immunize up to five mice, fusion, screening, subclone and initial characterization
With up to three monoclones and their supernatant for confirming detection
Generated antibody recognizes immunogen in ELISA assay
Antibody purification by affinity column (1-5mg) may be obtained upon request

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Custom Antibody Production Services

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Custom Antibody Production Services

Discuss your project requirements

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