Flow Cytometry Antibodies

Different cell populations can be distinguished from one another in a complex sample using flow cytometry antibodies conjugated to non-overlapping fluorophores.

RayBiotech is proud to offer RayBright^® dyes with excellent brightness, covering most of the common emission colors of similar dyes. The tables below provide the excitation and emission spectra of our RayBright fluorochromes and their equivalent dyes.

We offer a FREE trial size (5 µg) of any of our RayBright^® flow cytometry antibodies. For more information, e-mail our support team at [email protected].

RayBiotech Superior Fluorochromes	Excitation Laser	Max Excitation/Emission	Replacing dyes of equivalent wavelengths
RayBright® UV 450	UV 355	347/448 nm	Alexa Fluor 350
RayBright® Violet 450	Violet 407	403/454 nm	Pacific Blue, Alexa Fluor 450
RayBright® Violet 500	Violet 407	405/537 nm	Alexa Fluor 430, BV510
RayBright® Violet 600	Violet 407	407/600 nm	BD Brilliant Violet 605
RayBright® Violet 655	Violet 407	407/655 nm	BD Brilliant Violet 655
RayBright® Blue 488	Blue 488	491/518 nm	Alexa Fluor 488, FITC, BD BB515
RayBright® Blue/Green 575	Blue 488/Green 561	505/570, 553/570 nm	R-PE, Cy3
RayBright® Green 594	Green 561	594/614 nm	PE-Texas Red, PE-CF594, PE-eFluor610
RayBright® Blue/Green 647 (PE-tandem dye)	Blue 488/Green 561	496/664, 565/664 nm	PE-Cy5
RayBright® Blue/Green 780 (PE-tandem dye)	Blue 488/Green 561	496/780, 565/780 nm	PE-Cy7, PE-Alexa Fluor 750
RayBright® Red 647	Red 640	649/664 nm	APC, Alexa Fluor 647, Cy5
RayBright® Red 700	Red 640	685/710 nm	Alexa Fluor 700, APC-R700
RayBright® Red 780	Red 640	629/780 nm	Alexa Fluor 750, APC-eFluor 780

Traditional dyes	Excitation Laser	Max Excitation/Emission
PerCP	Blue 488	482/667 nm
R-PE	Blue 488/Green 561	496/780, 565/780 nm
APC	Red 640	651/662 nm

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RayBright^® Dye Labeling Results