RayBiotech’s stable cell line generation service uses a mammalian retrovirus to incorporate foreign genes into the host genome. This enables the gene to be passed to daughter cells and maintained without the use of antibiotics. Our comprehensive service cover every step of the workflow, from gene synthesis to cell culture and treatment.
Compared to transient gene expression, stable expression has the following advantages:
Protein localization of the foreign gene is visualized via the GFP fusion tag. Cell images using a A) GFP filter or B) DAPI filter. C) A and B images merged. 1) Only GFP was expressed; GFP did not localize to a specific cellular component. 2) GeneX-GFP localized to nucleoli within the nucleus. White bar = 10 µm. 3) GeneY-GFP localized to mitochondria within the cytoplasm.
Phase 1: Gene synthesis (usually 2-4 weeks)
The target gene is synthesized.
Phase 2: Gene cloning (2 weeks)
The gene-of-interest will be cloned into a retrovirus plasmid. Proper insertion of the gene is confirmed via DNA sequencing.
Phase 3: Retroviral packaging (2 weeks)
The retrovirus titer is determined using human HEK293 cells. Options for shipping include:
Phase 4: Retrovirus titering (2-3 weeks)
The retrovirus titer is determined using human HEK293 cells.
Phase 5: Stable cell line generation (1-2 months)
Cells are transduced with the gene-of-interest. Stable gene expression will be confirmed with a western blot.
Phase 6: Cell culture & treatment (depends on experiment)
We can culture and treat the stable cells according to your specifications. Data analysis included.
Leverage the same quality antibody production processes we put into our products for your custom research and bulk antibody production needs.
When you need a few extra hands to get an assay completed or support from experimental design to data analysis, our experienced team of scientists is ready to help.
GMP-certified proteins are meticulously manufactured under precise guidelines to guarantee product quality and consistency for safe and effective use in these applications.