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Cell Biology Services

RayBiotech provides integrated, end-to-end cell-based solutions—from cell culture and transfection to downstream protein and functional analysis. Our scientists design and execute customized in vitro studies that connect cellular biology with quantitative molecular readouts.

We support projects ranging from basic research through preclinical development, combining robust cell culture platforms with validated analytical assays such as ELISA, Western blot, multiplex immunoassays, and functional cell-based assays.

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In Vitro Cell Culture & Functional Assays
Cell & Tissue Processing
Exosome Analysis
Flow Cytometry

In Vitro Cell Culture & Functional Assays

Our dedicated cell biology team provides comprehensive cell culture services designed to generate reproducible, biologically relevant data.

Core Capabilities

  • 2D and 3D cell culture, cell expansion, and cryopreservation
  • Cell processing and sample aliquoting
  • Primary cell isolation and primary explant culture
  • Custom optimization of cell lines, including development of optimal media formulations and treatment regimens
  • Optimization of cell line expression systems
  • Stable and transient transfection (plasmid, siRNA, shRNA)
  • Generation of overexpressing and knockdown cell lines
  • Stable cell line development
  • Reporter gene cell lines and functional reporter assays
  • High-content image analysis and image-enabled cell line generation
  • Cell stimulation studies (cytokines, small molecules, biologics)
  • Cell surface assays and intracellular assays
  • Custom assay development for specific pathways or targets

Cell Migration, Invasion & Functional Assays

We offer validated functional assays to assess cellular behavior under defined experimental conditions.

  • Cell migration assays (wound healing / scratch assays)
  • Transwell migration assays
  • Matrigel invasion assays
  • Proliferation assays
  • Cytotoxicity and viability assays
  • Apoptosis assays
  • DNA damage and oxidative stress analysis
  • Immune cell activation and functional testing

Functional studies can be seamlessly integrated with ELISA, Western blot, or multiplex assays to generate complete mechanistic datasets.

Cell Culture + Downstream Protein Analysis

We bridge cell-based experiments with quantitative protein analysis to provide complete biological insight.

  • Cell Culture → ELISA
    • Quantification of secreted cytokines, chemokines, and growth factors
    • Time-course and dose-response studies
    • Biomarker validation and pathway analysis
    • Multiple ELISA platforms available, including sandwich ELISA, competitive ELISA, indirect ELISA, high-sensitivity qIPCR ELISA, and PTM-specific ELISAs
  • Cell Culture → Western Blot
    • Intracellular signaling pathway analysis
    • Phosphorylation and activation studies
    • Knockdown/overexpression confirmation
    • Apoptosis and stress response markers
  • Cell Culture → Multiplex Protein Profiling
    • Cytokine and inflammation panels
    • Oncology biomarker profiling
    • Immune response characterization
    • Pathway-focused antibody arrays

Cell & Tissue Processing

We provide comprehensive cell and tissue processing solutions to support research and translational studies. Our team manages the preparation, enrichment, and expansion of live cells to ensure high-quality inputs for downstream cell-based assays, protein analysis, and molecular workflows.

Capabilities

  • Live/dead cell separation
  • Magnetic cell sorting (positive and negative selection)
  • PBMC, macrophage, T cell, and B cell isolation and expansion
  • Sample preparation for downstream protein and molecular analysis


in vitro cell culture


Exosome Analysis

Our exosome services support advanced research applications by combining optimized isolation and characterization workflows with downstream molecular and protein analysis.

Capabilities

  • Exosome quantification and quality assessment
  • Surface marker profiling
  • Bead-based staining and analysis
  • Exosome production and purification

Learn More

Flow Cytometry

Flow cytometry services are also available as part of our integrated cell-based workflows:

  • Immunophenotyping
  • Intracellular staining
  • Cell surface marker analysis
  • Cell proliferation
  • Cell cycle analysis and apoptosis
  • Cellular activation
  • Cell toxicity and DNA damage
  • Phospho-flow
  • Immune checkpoint profiling
  • Functional immune assays (ADCC, ADCP, CDC)
  • Cytokine storm and comprehensive protein profiling

Learn More

Discuss your project requirements

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FAQ

Frequently Asked Questions

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Fill out the service form and include it with your shipment to RayBiotech. Cells should be in the appropriate amount of dimethyl sulfoxide (DMSO) and shipped frozen (on dry ice).

See also our Sample Shipment Instructions.

  1. Aliquot the cells into cryovials containing cell culture media and dimethyl sulfoxide (DMSO) at the desired cell concentration. The appropriate amount of DMSO depends on the cells, but is usually 5 - 10% (v/v).
  2. Slowly freeze cells to maintain cell viability. You can either place the cryovials in a controlled rate freezing apparatus (decreasing the temperature approximately 1°C per minute) or in an isopropanol chamber overnight at -80°C.
  3. Store the cells in the gas phase in a liquid nitrogen tank.
  4. Ship the cells on dry ice. Ensure that there is enough dry ice to keep the cells frozen during shipping. Please ship your samples overnight on Monday through Wednesday only. Learn more about how to ship samples by clicking the SAMPLE SHIPMENT INSTRUCTIONS button below.

Still have questions?

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Related Services

Stable Cell Line Generation Service

RayBiotech’s stable cell line generation service uses a mammalian retrovirus to incorporate foreign genes into the host genome. From gene synthesis to cell culture and treatment.

Flow Cytometry Service

RayBiotech provides a comprehensive Flow Cytometry Full Testing Service to evaluate cellular and signaling phenotypes, cell growth

Related Products

Cell-Based ELISA Kits

Cell-Based Protein Phosphorylation ELISA kits are rapid assays which can be used for measuring the relative amount of protein phosphorylation and monitoring the effect of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured cell lines.

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