RayBio® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Number of Targets Detected | 62 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Species | Mouse |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Axl
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
|
| CRG-2
| CTACK (CCL27)
| CXCL16
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
| GCSF
| GM-CSF
| IFN-gamma
|
| IGFBP-3
| IGFBP-5
| IGFBP-6
| IL-1 beta (IL-1 F2)
| IL-10
|
| IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-17A
| IL-1 alpha (IL-1 F1)
|
| IL-2
| IL-3
| IL-3 R beta
| IL-4
| IL-5
|
| IL-6
| IL-9
| KC (CXCL1)
| Leptin
| Leptin R
|
| LIX
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-5
|
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
|
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
| Platelet Factor 4 (CXCL4)
| P-Selectin
| RANTES (CCL5)
|
| SCF
| SDF-1 alpha (CXCL12 alpha)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TARC (CCL17)
|
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TIMP-1
| TNF alpha
| Thrombopoietin (TPO)
|
| VCAM-1 (CD106)
| VEGF-A
| |||
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Pelaez-Garcia A., Barderas R., Batlle R., et al. A proteomic analysis reveals that Snail regulates the expression of the nuclear orphan receptor Nuclear Receptor Subfamily 2 Group F Member 6 (Nr2f6) and interleukin 17 (IL-17) to inhibit adipocyte differentiation. Mol Cell Proteomics. 2015 Feb;14(2):303-15. doi: 10.1074/mcp.M114.045328.
Species:
Mouse
Sample Type:
Conditioned Media (3T3-L1 or mMSC cells)
Fuster-Matanzo, Almudena, et al. "Dual effects of increased glycogen synthase kinase-3? activity on adult neurogenesis." Human molecular genetics 22.7 (2012): 1300-1315.
Species:
Mouse
Sample Type:
Tissue Lysate (Hippocampi of Gene-ON and doxycycline-treated (Gene-OFF) Tet/GSK3b mice)
Nair, Ashwin, et al. "Biomaterial implants mediate autologous stem cell recruitment in mice." Acta biomaterialia 7.11 (2011): 3887-3895.
Species:
Mouse
Sample Type:
Tissue Lysate (skin)
Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9;479(7374):547-51. doi: 10.1038/nature10599.
Species:
Mouse
Sample Type:
Conditioned Media (hepatocytes)
Moon H., Donahue L., Choi E., et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell . 2017 Nov 2;21(5):665-678.e6. doi: 10.1016/j.stem.2017.09.001. Epub 2017 Oct 12.
Species:
Mouse
Sample Type:
Conditioned Media (Mouse Skin Drosal cells Melanocyte Stem cell translocation media)
Hughes, David J., et al. "Chemokine binding protein M3 of murine gammaherpesvirus 68 modulates the host response to infection in a natural host." PLoS Pathog 7.3 (2011): e1001321.
Species:
Mouse
Sample Type:
Tissue lysate (lung)
Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9;479(7374):547-51. doi: 10.1038/nature10599.
Species:
Mouse
Sample Type:
Tissue Lysate (whole liver Lysate)
Vergori L., Lauret E., Gaceb A., et al. PPAR? Regulates Endothelial Progenitor Cell Maturation and Myeloid Lineage Differentiation Through a NADPH Oxidase-Dependent Mechanism in Mice. Stem Cells. 2015 Apr;33(4):1292-303. doi: 10.1002/stem.1924.
Species:
Mouse
Sample Type:
Conditioned Media (BM dervied cells treated with M-CSF)
Simon M., Ven Meter M., Ablaeva J., et al. LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation. Cell Metab . 2019 Apr 2;29(4):871-885.e5. doi: 10.1016/j.cmet.2019.02.014. Epub 2019 Mar 7.
Species:
Mouse
Sample Type:
Plasma (nucleoside reverse-transcriptase inhibitors (D4T treatment))
Szabolcsi V., Celio M. De novo Expression of Parvalbumin in Ependymal Cells in Response to Brain Injury Promotes Ependymal Remodeling and Wound Repair. GLIA 2014, epub ahead of print. DOI: 10.1002/glia.22768
Species:
Mouse
Sample Type:
Conditioned Media (Hippocampal isolated cells.)
- Great productI used this array to analyze some astrocyte culture-conditioned (serum-free) media (about 1 million cells/well). I didn't use the software for analysis, and it took me about a day to analyze the four membranes. I didn't dilute the media and I certainly wasn't maxing out the signal when going with all of the optional overnight incubations.
from Northwestern University,
on
- Great productProtocol is easy to follow and convenient to use. It is time-saving.
from Purdue University,
on
- Great productI used this array in the serum samples of deer mice and got good pictures. The images can be easily analyzed by Imagelab.
from University of South Carolina,
on
- Great productThis product is fantastic! We use to analyze the connection between irradiation dose and inflammation in mouse plasma in mice with different genetic backgrounds. The protocol is easy to follow and the results are always clear to interpret.
from Roswell Park Cancer Institute,
on
- Great productWe used the AAM-CYT-3-8 Kit. With the detailed protocol manual, we could take all the necessary precautions to get consistent signals with the positive controls and we could confirm the same after repeating it for the selected individual cytokines that were deferentially unregulated
from W.M. Keck Center for Transgene Research,
on
- Great productWe used the above kit to get the cytokine patterns in the secretome of the infected bone marrow derived macrophages. The kit seems to be consistent. With in vitro stimulated cells, the cytokine levels were found. We used undiluted samples in analysis. The FBS from VWR at 2% was also present in the medium.
from University of Chicago,
on
- Great productThe protocol is easy and user friendly. However, the read out proved difficult as the radiance between samples in different conditions can not be discerned clearly even when using advanced western blot readout software. Tried different exposures and did not get adequate results for a manuscript figure.
from National Cancer Institute, NIH,
on
- Great productWe used the assay to analyze lung tissue extracts at 500ug per membrane. We got a good signal and reproducible results. However, I would recommend using different ECL reagent for detection, the one provided with the kit is not the best.
from University of Kansas Medical Center,
on
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