Multiplex ELISA array detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Number of Targets Detected | 40 |
| Research Area | Inflammation |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| More Information | Suggested Application Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range |
| Storage / Stability |
|
| Species | Human |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BLC (CXCL13)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
|
I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 ra (IL-1 F3)
| IL-2
| IL-4
| IL-5
| IL-6
|
IL-6 R
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-11
|
IL-12 p40
| IL-12 p70
| IL-13
| IL-15
| IL-16
|
IL-17A
| MCP-1 (CCL2)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| PDGF-BB
| RANTES (CCL5)
| TIMP-1
|
TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
Species:
Human
Sample Type:
Vitreous Fluid (Vitreous sample from retinopathy patients)
Liao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907.
Species:
Human
Sample Type:
Conditioned Media (Umbilical cord stem cells)
Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
Species:
Human
Sample Type:
Vitreous Fluid (Human Vitreous Fluid samples)
Nacarelli T., Lau L., Fukumoto T., et al. NAD+ metabolism governs the proinflammatory senescence-associated secretome. Nat Cell Biol . 2019 Mar;21(3):397-407. doi: 10.1038/s41556-019-0287-4. Epub 2019 Feb 18.
Species:
Human
Sample Type:
Conditioned Media (IMR90 Fibroblasta and cocultures)
Liu P., Li F., Lin J., et al. m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype. Nature Cell Biology volume 23, pages 355-365 (2021)
Species:
Human
Sample Type:
Conditioned Media (IMR90 cells Oncology in vitro culture with RAS induced senescence)
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
Species:
Human
Sample Type:
Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094
Species:
Human
Sample Type:
Conditioned Media (Neutrophil swarming experiments)
Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.
Species:
Human
Sample Type:
Plasma (Polycyclic aromatic hydrocarbon exposed patients)
Napolioni, Valerio, et al. "Plasma cytokine profiling in sibling pairs discordant for autism spectrum disorder." Journal of neuroinflammation 10.1 (2013): 813.
Species:
Human
Sample Type:
Plasma (autism )
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327
Species:
Human
Sample Type:
Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)
- Great productThis is our third plate. It is a little bit of work, too much washing though, but once you get use of it, it's pretty easy forward. I'd recommend it.
from Stanford University,
on
- Great productWe used QAH-INF-3 product for plasma samples. Method was very easy to perform as well we used their free scanning service, it was quick.The technical support is also helpful and quick. Overall we liked product as well service.We are planning to buy more.
from Texas tech university health science cen,
on
- Great productWe used QAH-INF-3 to measure cytokine in the human plasma sample. The protocol is very simple and easy to perform. We used RayBiotech's free scanning service which was completed within a week. They have very good resources for analyzing data and a very responsive team.We really like this product and plan to use RayBiotech's service/products in future assays.
from Texas Tech University Health Science Center,
on
- Great productIt's good and easy to use, but need a laser scanner.
from University of South Florida,
on
- Great productMy lab members have used this array several times before, they recommend it to me, both mouse's and human's are very reliable!
from The Wistar Institute,
on
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