Product Description

Specifications

Size	100 µg, 250 µg
Estimated Lead Time	1-2 business days
Species	SARS-CoV-2
Accession Number	QHD43416
Protein Name / Synonyms	SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein
Expressed Region	Val16 - Gln690
Expression System	E. coli
Tag	N-terminal histidine tag
Purity	>80 %
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	~75 kDa
Format	Liquid
Formulation	Filtered solution in 50 mM Tris-HCl, 500 mM NaCl, 600 mM arginine, 2 mM oxidized glutathione, 4 mM reduced glutathione, 0.2% PEG4000, 10% glycerol, 2 mM DTT, 20 mM Hepes (pH 9).
Concentration (lot specific)	Lot specific (see the label on the vial), determined by Bradford protein assay.
Research Area	Infectious Disease, COVID-19
Shipping Type	Blue ice
Storage	-20°C

SDS-PAGE Image

Storage/Stability

Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Introduction

The SARS-CoV-2 spike (S) protein is a surface glycoprotein that mediates viral entry into host cells. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for recognizing and binding to the ACE2 receptor. This interaction is the first step in viral infection, making the S1 subunit a critical focus for vaccine development and antiviral therapies.

Reference: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03143-7

Typical Data

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) induced IL-6 expression, IL-6 secretion, and STAT3 phosphorylation in brain endothelial cells. Figure adapted from Stangis et al., NeuroImmunePharmacol. Ther., 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used as an ELISA antigen to evaluate vaccine-induced IgG responses in a multi-epitope immunization study. The study also included our S2 protein (Cat# 230-01103), along with additional RayBiotech viral antigens, to assess broader humoral immunity. Figure adapted from Atanasova et al., Pharmaceuticals, 2024.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to coat ELISA plates for detecting spike-specific IgG responses in a phase I COVID-19 vaccine trial. Shown here: IgG levels after high-dose intramuscular vaccination. Figure adapted from Ponce-de-León et al., npj Vaccines, 2023.

RayBiotech's SARS-CoV-2 S1 protein (Cat# 230-01101) was used to stimulate endothelial cells, triggering inflammatory responses including IL-6 (shown here) and MCP-1 upregulation. Figure adapted from Montezano et al., Scientific Reports, 2023.

RayBiotech’s recombinant spike proteins and binding assay kit were used to assess ACE2-binding inhibition by viral spike domains. Shown here: inhibition of ACE2–RBD binding by SARS-CoV-2 S1 (Cat# 230-01101), SARS-CoV-2 RBD (Cat# 230-01102), and other viral proteins using the Spike–ACE2 Binding Assay Kit I (Cat# CoV-SACE2). Figure adapted from Bejoy et al., Arch BiochemBiophys, 2023.

SDS-PAGE Image

Storage/Stability

Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Introduction

Reference: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03143-7

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Typical Data

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Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Recombinant SARS-CoV-2 Spike Protein, S1 Subunit

Product Description

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Introduction

Typical Data

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Recombinant SARS-CoV-2 Spike Protein, S1 Subunit