1-888-494-8555

5% Off All Online Orders

ELISA Kits

Superior Quality ELISA Kits Guaranteed

Blue Ribbon SealRayBiotech manufactures over 3,000 ISO certified and rigorously tested ELISA kits, all backed by our 90-day 100% satisfaction guarantee. You can evaluate our kits with no risks, no worries. All RayBio® ELISA kits are manufactured in the USA and validated for exceptional reproducibility. Learn about our available ELISA GLP testing services.

 

Sandwich ELISA


Sandwich ELISA Detection
Advantage High specificity and sensitivity; Thousands of targets available
Assay Principle Sandwich-based (matched antibody pairs)
Targets Detected Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types Plasma, serum, cell culture media, lysates, clarified body fluids
Application Protein quantification; Validation of array results; Single target ELISA
Species Human | Mouse | Rat | Cow | Dog | Horse | Cat | Sheep | Pig | Rabbit | Rhesus macaque | Cotton rat

Learn MoreView TargetsBrowse Sandwich ELISAs

 

Phosphorylation ELISA


HSP27 (S82) Phospho-ELISA
Advantage Measure phosphorylated protein and pan protein in one experiment; Screen different lysates without performing a Western Blot analysis
Assay Principle Sandwich-based (matched antibody pairs)
Targets Detected Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules
Sample Types Cell & tissue lysates
Application Phospho-protein detection; phosphorylation pathway analysis
Species Human | Mouse | Rat

Learn MoreView TargetsBrowse Phospho-ELISAs

 

Transcription Factor Activity Assay


Transcription Factor ELISA Detection
Advantage Measure active transcription factors in nuclear extracts and cell lysates; Perfect alternative to EMSA
Assay Principle Direct (Transcription Factor-DNA Complex)
Targets Detected Active transcription factors
Sample Types Nuclear extracts, whole cell lysates
Application Semi-quantitative detection of transcription factor-DNA complexes in nuclear extracts
Species Human

Learn MoreView TargetsBrowse TF Assays

 

EIA


EIA Detection
Advantage Detect peptide hormones and other small molecules for which no antibody pair exists
Assay Principle Competition-based
Targets Detected Adipokines, peptide hormones
Sample Types Plasma, serum, cell culture media, lysates, clarified body fluids
Application Protein quantification; Validation of array results; Single target ELISA
Species Multi-species detection (all kits): human, mouse, rat

Learn MoreView TargetsBrowse EIAs

 

Cell-Based Phosphorylation ELISA


Cell-Based ELISA Assay Principle
Advantage Detect phosphorylated proteins in adherent cell lines; Eliminate tedious steps in preparing cell lysate and performing Western Blot.
Assay Principle Direct
Targets Detected Phosphorylated EGFRs, JNK, ERK, STATs, Phosphotyrosine, and other signaling molecules
Sample Types Adherent cell lines
Application Phospho-protein detection; phosphorylation pathway analysis
Species Human | Mouse | Rat

Learn MoreView TargetsBrowse Cell-Based ELISAs

 

SpeedELISA


ELISA Standard Curve
Advantage Three hour total processing time
Assay Principle Compressed work flow, Sandwich-based (matched antibody pairs)
Targets Detected Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types Plasma, serum, cell culture media*
Application Cytokine quantification; Validation of array results; Single target ELISA
Species Human | Mouse | Rat

Learn MoreView TargetsBrowse SpeedELISAs

 

IQELISA


IQELISA Detection
Advantage Extremely sensitive (10X more sensitive than traditional ELISA); Low sample volume (1/10th sample volume of traditional ELISA)
Assay Principle Sandwich-based (matched antibody pairs)
Targets Detected Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types Plasma, serum, cell culture media
Application Protein quantification; Validation of array results; Single target ELISA
Species Human

Learn MoreView TargetsBrowse IQELISAs

Comparison of ELISA Platforms

NameDesign PrincipleResult OutputSample TypesTargets DetectedSpecies Detected
Sandwich ELISA Sandwich-based Quantitative Plasma, serum, cell culture media, lysates, clarified body fluids Cytokines, growth factors, soluble receptors, and other soluble proteins H, M, R, B, C, E, F, G, P, L, N, S
Phosphorylation ELISA Sandwich-based Semi-Quantitative Cell & tissue lysates Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules H, M, R
Transcription Factor Activity Assay Direct (Transcription Factor-DNA Complex) Semi-Quantitative Nuclear extracts, whole cell lysates Active transcription factors H, M
EIA Competition-based Quantitative Plasma, serum, cell culture media, lysates, clarified body fluids Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules H, M, R
Cell-Based Phosphorylation ELISA Direct Semi-Quantitative Adherent cell lines Phosphorylated EGFRs, JNK, ERK, STATs, Phosphotyrosine, and other signaling molecules H, M, R
SpeedELISA Sandwich-based (compressed work flow) Quantitative Plasma, serum, cell culture media Cytokines, growth factors, soluble receptors, and other soluble proteins H, M, R
IQELISA Sandwich-based (PCR) Quantitative Plasma, serum, cell culture media Cytokines, growth factors, soluble receptors, and other soluble proteins H

*H=human  |  M=mouse  |  R=rat  |  P=porcine  |  C=canine  |  F=feline  |  B=bovine  |  E=equine  |  N=rhesus monkey  |  L=rabbit