ELISA Kits

ELISA kits are enzyme-linked immunosorbent assays designed for the quick detection and quantitation of target proteins, antibodies, and other soluble targets of interest. RayBiotech's objective is to manufacture a diverse selection of high quality ELISA kits to serve a broad range of research interests. Our target protein selection includes a wide variety of soluble proteins (cytokines, growth factors, signaling molecules), as well as transcription factors and post-translational modifications. We strive for species diversity as well, with 17 target species including rabbit, dog and zebrafish.

Sandwich ELISA kits utilize matched antibody pairs for a "sandwich-based" quantitative detection of cytokines, growth factors, receptors and other proteins. RayBiotech manufactures over 3,700 sandwich ELISA kits covering 17 different species. The 96-well plates come pre-coated with the specific capture antibodies for excellent reproducibility. Compatible sample types include plasma, serum, cell culture media, lysates, clarified body fluids

Features

• Pre-coated 96 well plate
• High specificity & sensitivity
• Thousands of choices
• Many compatible sample types
• Easy to use
• Reproducible and reliable

An indirect Enzyme-Linked Immunosorbent Assay (ELISA) is a common biochemical technique that is most suitable for determining total antibody concentrations in a sample. This method is commonly utilized to diagnose infection and to quantify antibodies against the invading antigen.

Features

• High sensitivity assays for COVID-19 proteins
• Validated with positive patients confirmed with PCR
• Results within 3 hours
• Compatible with serum, plasma, and dried blood samples

Phosphorylation ELISA kits are sandwich-based ELISAs specificly made for measuring phosphorylated proteins, such as MAPK, AKT, EGFR, STAT, mTOR, and other signaling molecules. This allows the researcher to screen numerous different cell lysates without performing Western Blot analyses. For normalization purposes, these kits can also measure phosphorylated protein and pan protein in one experiment. RayBiotech manufactures over 300 phospho-ELISAs for human, mouse, and rat species.

Features

• Verify pathway activation
• Minimal hands-on time, convenient
• Cost effective
• High sensitivity
• Non-radioactive material

IQELISA™ is an immuno-PCR assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR.

Features

• 10x more sensitive than traditional ELISA
• 1/10^th the sample volume of traditional ELISA
• Pre-coated 96 well plate
• Optimized for serum, plasma, urine and cell culture medium
• Reproducible and reliable

Competitive ELISA kits utilize the principle of competitive enzyme immunoassay (EIA), in which the target protein and a biotin-conjugated peptide bind competitively to a capture antibody. This method is effective for detecting peptide hormones and other molecules for which no antibody pair has been developed.

Features

• Quantitatively measure targets for which no antibody pair exists
• Broad species reactivity
• Optimized for serum, plasma, and cell culture medium
• Reproducible and reliable

Cell-Based ELISA kits utilize the direct design principle to measure the relative amount of protein phosphorylation and monitor the effect of various treatments, inhibitors, or activators in cultured cell lines.

Features

• Two 96 well tissue culture plates (12x8 well) for 192 Assay
• Eliminate steps in preparing cell lysate and performing Western Blot
• Work reliably with wide range of cell lines
• High sensitivity and simple procedure
• Non-radioactive materials

Transcription Factor-Enzyme Linked Immunosorbent Assays are non-radioactive assays to semi-quantitatively measure active transcription factors in nuclear extracts and cell lysates. They offer an easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.

Features

• Perfect alternative to EMSA
• Specific transcription factor-DNA binding assay
• Easy to perform in an ELISA format
• Non-radioactive materials
• Compatible with nuclear extracts, whole cell lysates

RayBiotech offers binding assays for studying receptor-ligand or drug-target interactions. Using an ELISA-like format, the protein or small molecule of interest is coated on a 96-well microplate and potential inhibitors are then added in the presence of the interacting molecule. This assay enables rapid, high throughput, in-vitro screening of potential inhibitors. Browse ready-made kits to query Spike-receptor interactions that facilitate SARS-CoV-2 viral entry into host cells, as well as to detect drug antibodies (DA) and anti-drug antibodies (ADA). 

Features

• Simple to use
• Affordable screening option
• High throughput
• Rapid: Get results in 5 hours!
• Compatible with various sample types
• Handled safely at biosafety level 1 (BSL1) (BSL requirements may change based on the sample type being tested.)

Utilize the sandwich-based principle of detection, with a compressed work flow that allows quantitative measurement of protein concentration in only three hours.

Features

• Compressed workflow with only one wash step and three incubation steps
• Only 3 hours total processing time
• Strip plate format minimizes waste
• Complete kit includes all necessary reagents
• Highly sensitive and specific
• Reproducible and reliable
• Optimized for serum, plasma, urine and cell culture medium
• Excellent for follow up/confirmation of antibody array data