ELISA Kits

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RayBiotech manufactures over 3,000 ISO certified and rigorously tested ELISA kits, all backed by our 90-day 100% satisfaction guarantee. You can evaluate our kits with no risks, no worries. All RayBio^® ELISA kits are manufactured in the USA and validated for exceptional reproducibility. Learn about our available ELISA GLP testing services.

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Sandwich ELISA

Advantage	High specificity and sensitivity; Thousands of targets available
Assay Principle	Sandwich-based (matched antibody pairs)
Targets Detected	Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types	Plasma, serum, cell culture media, lysates, clarified body fluids
Application	Protein quantification; Validation of array results; Single target ELISA
Species	Human \| Mouse \| Rat \| Cow \| Dog \| Horse \| Cat \| Sheep \| Pig \| Rabbit \| Rhesus macaque \| Cotton rat

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Phosphorylation ELISA

Advantage	Measure phosphorylated protein and pan protein in one experiment; Screen different lysates without performing a Western Blot analysis
Assay Principle	Sandwich-based (matched antibody pairs)
Targets Detected	Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules
Sample Types	Cell & tissue lysates
Application	Phospho-protein detection; phosphorylation pathway analysis
Species	Human \| Mouse \| Rat

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Transcription Factor Activity Assay

Advantage	Measure active transcription factors in nuclear extracts and cell lysates; Perfect alternative to EMSA
Assay Principle	Direct (Transcription Factor-DNA Complex)
Targets Detected	Active transcription factors
Sample Types	Nuclear extracts, whole cell lysates
Application	Semi-quantitative detection of transcription factor-DNA complexes in nuclear extracts
Species	Human

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EIA

Advantage	Detect peptide hormones and other small molecules for which no antibody pair exists
Assay Principle	Competition-based
Targets Detected	Adipokines, peptide hormones
Sample Types	Plasma, serum, cell culture media, lysates, clarified body fluids
Application	Protein quantification; Validation of array results; Single target ELISA
Species	Multi-species detection (all kits): human, mouse, rat

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Cell-Based Phosphorylation ELISA

Advantage	Detect phosphorylated proteins in adherent cell lines; Eliminate tedious steps in preparing cell lysate and performing Western Blot.
Assay Principle	Direct
Targets Detected	Phosphorylated EGFRs, JNK, ERK, STATs, Phosphotyrosine, and other signaling molecules
Sample Types	Adherent cell lines
Application	Phospho-protein detection; phosphorylation pathway analysis
Species	Human \| Mouse \| Rat

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SpeedELISA

Advantage	Three hour total processing time
Assay Principle	Compressed work flow, Sandwich-based (matched antibody pairs)
Targets Detected	Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types	Plasma, serum, cell culture media*
Application	Cytokine quantification; Validation of array results; Single target ELISA
Species	Human \| Mouse \| Rat

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IQELISA

Advantage	Extremely sensitive (10X more sensitive than traditional ELISA); Low sample volume (1/10th sample volume of traditional ELISA)
Assay Principle	Sandwich-based (matched antibody pairs)
Targets Detected	Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types	Plasma, serum, cell culture media
Application	Protein quantification; Validation of array results; Single target ELISA
Species	Human

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Comparison of ELISA Platforms

Name	Design Principle	Result Output	Sample Types	Targets Detected	Species Detected
Sandwich ELISA	Sandwich-based	Quantitative	Plasma, serum, cell culture media, lysates, clarified body fluids	Cytokines, growth factors, soluble receptors, and other soluble proteins	H, M, R, B, C, E, F, G, P, L, N, S
Phosphorylation ELISA	Sandwich-based	Semi-Quantitative	Cell & tissue lysates	Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules	H, M, R
Transcription Factor Activity Assay	Direct (Transcription Factor-DNA Complex)	Semi-Quantitative	Nuclear extracts, whole cell lysates	Active transcription factors	H, M
EIA	Competition-based	Quantitative	Plasma, serum, cell culture media, lysates, clarified body fluids	Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules	H, M, R
Cell-Based Phosphorylation ELISA	Direct	Semi-Quantitative	Adherent cell lines	Phosphorylated EGFRs, JNK, ERK, STATs, Phosphotyrosine, and other signaling molecules	H, M, R
SpeedELISA	Sandwich-based (compressed work flow)	Quantitative	Plasma, serum, cell culture media	Cytokines, growth factors, soluble receptors, and other soluble proteins	H, M, R
IQELISA	Sandwich-based (PCR)	Quantitative	Plasma, serum, cell culture media	Cytokines, growth factors, soluble receptors, and other soluble proteins	H