Multiplex Assays

Q: What is the difference between an antibody array and a protein array?

Protein arrays use immobilized recombinant proteins on a solid support to profile serological antibodies as well as to study protein interactions with other proteins, peptides, DNA, and drugs. Antibody arrays, on the other hand, use immobilized antibodies on a solid support and are used for protein expression profiling.

Q: Do I need any special equipment for multiplex assays?

Not when you work with RayBiotech! While our glass side arrays do require a laser scanner for fluorescent detection, we offer a free scanning service. Simply send us your slides and we’ll send you your results. To see if your laser scanner is compatible, click here.

Multiplex assays (also known as 'arrays') are immunoassays that allow simultaneous detection of multiple analytes in a single experiment. The analytes can include proteins (cytokines, peptides, growth hormones, etc.), antibodies, nucleic acids, or other molecules. Using a multiplex assay can be much more efficient than a singleplex immunoassay, such as ELISA, saving both time and resources.

Move quickly while gaining comprehensive insights into the composition of your samples with high-quality multiplex assays from RayBiotech. Screen up to 8,000 biomarkers in one experiment using our proteome profiling arrays. Or utilize a more targeted approach using arrays with target panels designed for specific research areas. Confidently profile cytokines and auto-antibodies, identify glycan binding partners, analyze isotype antibodies, and more when you choose multiplex assays from the most experienced company in the business.

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Multianalyte Immunodetection For Biomarker Exploration

Protein biomarkers provide insight into important biological processes such as disease progression and the therapeutic efficacy of a treatment. However, because these processes are so complex, it’s crucial to analyze multiple biomarkers simultaneously using multiplex assays such as antibody arrays, phosphorylation arrays, and bead arrays rather than single-analyte immunoassays.

With over 700 ISO certified and rigorously tested multiplex assays, you’ll be able to find what you need to address your research objectives. Because all our arrays include fast shipping, are made in the USA, and produce reproducible results, you’ll be able to discover faster and with confidence. And if you ever need a hand, our team of PhD scientists are here to answer your questions, whether they’re about the science, troubleshooting an experiment, or discussing your options for RayBiotech’s services. Our goal is to help you succeed.

Types of Multiplex Assays

Cytokine Arrays

Deepen your understanding of changing cytokine levels with the speed and certainty that only multiplex analysis can deliver.

Antibody Arrays

Build a comprehensive view of protein expression levels with quantitative and semi-quantitative multiplex antibody arrays.

Proteome Profiling Arrays

Glass slide microarrays designed to comprehensively analyze, screen, and measure thousands of proteins simultaneously in a small sample volume.

Cytometric Bead Arrays (CBA)

Use RayPlex^® bead-based multiplex assays with any standard flow cytometer and RayBiotech’s validated antibody pairs.

Phosphorylation Arrays

Investigate the regulation of cellular processes, including cell cycle, cell growth, and cell apoptosis.

Protein Arrays

Screen for protein-protein and protein-ligand interactions to profile autoantibodies, identify receptors, and more.

Peptide Arrays

Peptide epitope mapping arrays suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc.

PTM Arrays

Post-Translational Modification (PTM) arrays for multiplex detection of Acetylation, Acylation, Nitration, Nitrosylation, Oxidation, and SUMOylation.

Glycobiology Arrays

Gain insight into disease processes by profiling glycosylation with our versatile and innovative products.

Arrays by Research Focus

Angiogenesis Arrays

Apoptosis Arrays

Autophagy Arrays

Bone Metabolism Arrays

Chemokine Arrays

Disease Focused Arrays

DNA Damage Arrays

ECM Arrays

Growth Factor Arrays

Inflammation Arrays

Isotyping Arrays

Neuroscience Arrays

Pathway Arrays

Stem Cell Arrays

T Cell Arrays

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	Antibody Arrays	Protein Arrays	Phosphorylation Arrays	Glycobiology Arrays
Targets Detected	Proteins (cytokines, chemokines, growth factors, soluble receptors, etc.)	Auto-antibodies or protein interactions	Adipokines, peptide hormones	Glycans, lectins, sugar binding moieties
Immobilized Molecule	Antibodies	Proteins	Antibodies	Glycans, Antibodies, Lectins
Sample Types	Plasma, serum, cell culture media, lysates, body fluids, conditioned media	Serum, Plasma, Cell and tissue lysates, Cell Culture Supernatants, Proteins, Hydrothorax, Antibodies, Small Molecules	Plasma, serum, cell culture media, lysates, clarified body fluids	Serum, Plasma, Cell Culture Supernatants, Cell and Tissue Lysates, Purified Proteins
Application	Protein expression profiling, biomarker discovery	Auto-antibody profiling, characterizing antibody specificity, can be adapted for detection of protein-protein interactions, protein modifications, small molecule-protein interaction	Cytokine quantification; Validation of array results; Single target ELISA	Determine glycoprotein profile of sample, find glycan binding partners, determine glycan profile

Frequently Asked Questions

Still have questions?

What is the difference between an antibody array and a protein array?

What is a multiplex ELISA?

A multiplex ELISA uses ELISA sandwich-based immunoassay technology and applies it to beads, allowing for the simultaneous measurement of multiple analytes in a sample.

In a multiplex assay, fluorescent beads are pre-coated with capture antibodies. As the beads incubate with a sample, proteins bind to the corresponding capture antibody bead. Detection antibodies allow a flow cytometer to measure the presence of protein within a sample.

Do I need any special equipment for multiplex assays?

Related Services

Biostatistics and Bioinformatics

Whether you’re short on analytical resources or expertise, our experienced team can support your studies with a wide range of biostatistics and bioinformatics services.

Array Scanning and Analysis

Conserve precious sample while obtaining comprehensive protein expression insights even if you don’t have access to a laser scanner—scanning is free for all RayBio glass slide arrays.

Array Printing Service

We can print an array of any biomolecule, including antibodies, proteins, glycoproteins, glycolipids, complex biological fluids, peptides, or other small molecules.


	Antibody Arrays	Protein Arrays	Phosphorylation Arrays	Glycobiology Arrays
Targets Detected	Proteins (cytokines, chemokines, growth factors, soluble receptors, etc.)	Auto-antibodies or protein interactions	Adipokines, peptide hormones	Glycans, lectins, sugar binding moieties
Immobilized Molecule	Antibodies	Proteins	Antibodies	Glycans, Antibodies, Lectins
Sample Types	Plasma, serum, cell culture media, lysates, body fluids, conditioned media	Serum, Plasma, Cell and tissue lysates, Cell Culture Supernatants, Proteins, Hydrothorax, Antibodies, Small Molecules	Plasma, serum, cell culture media, lysates, clarified body fluids	Serum, Plasma, Cell Culture Supernatants, Cell and Tissue Lysates, Purified Proteins
Application	Protein expression profiling, biomarker discovery	Auto-antibody profiling, characterizing antibody specificity, can be adapted for detection of protein-protein interactions, protein modifications, small molecule-protein interaction	Cytokine quantification; Validation of array results; Single target ELISA	Determine glycoprotein profile of sample, find glycan binding partners, determine glycan profile

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Multiplex Assays

Multianalyte Immunodetection For Biomarker Exploration