Isotype Control Antibodies
Isotype control antibodies use the same species and isotype as the primary antibody and are used as negative controls to help researchers determine how much of the observed signal is due to specific binding of the primary antibody or non-specific binding, including background noise. For example, if a researcher is using a mouse IgG1 antibody to detect a specific protein of interest, they would use a mouse IgG1 isotype control antibody to determine the background binding of the primary antibody. The isotype control antibody should not bind to the protein of interest and should be used at the same concentration as the primary antibody.
RayBiotech provides a diverse selection of various isotope controls for researchers to effectively determine specific binding vs. non-specific binding, to assess background noise, and to ensure reproducibility.
- Specific vs. non-specific binding: Determine if any observed signal is due to specific binding of the primary antibody or non-specific binding.
- Assess background noise: Determine the level of background noise to help detect low levels of a protein.
- Ensure reproducibility: Confirm results are reproducible between experiments and labs.