Cytometric Bead Arrays: RayPlex®
Take advantage of cytometric bead array technology for solution-based high throughput protein detection using a standard flow cytometer.
What Are Cytometric Bead Arrays?
Cytometric bead arrays (CBA) are sandwich immunoassays that use a capture antibody immobilized on the bead to detect a target protein, and a fluorescently-labeled detection antibody to visualize the captured target protein. By using different combinations of microbead sizes and fluorophores, researchers can simultaneously analyze multiple target proteins.
RayPlex cytometric bead arrays are compatible with most standard flow cytometers that are equipped with blue and red lasers capable of detecting fluorescence in the phycoerythrin (PE) and allophycocyanin (APC) channels, respectively.
Armed with the precision that comes with sandwich immunoassay technology and the measurement capabilities of multiplex bead arrays, you can discover more deeply and more quickly with RayPlex.
How Do Cytometric Bead Arrays Work?
- Incubate your sample with the RayPlex beads. The proteins will bind to the corresponding pre-coated capture antibody bead.
- Detect bound target by adding biotinylated detection antibodies.
- Wash away unbound protein and add PE-conjugated streptavidin.
- Analyze beads with a flow cytometer.
- Process and interpret the flow cytometry data.
What Research Applications Are Cytometric Bead Arrays Used For?
We’ve seen the RayPlex cytometric bead arrays used to address a large range of research questions. Here are a few of our favorite applications:
- High-throughput profiling of cytokine expression.
- Validation of semi-quantitative antibody array results.
- Identifying potential molecular targets for drug development.
- Identifying the molecular mechanisms of drug action.
- Identifying crucial factors involved in disease processes.
- Discovering biomarkers for disease management.
- Discovering expression patterns for molecular classification of diseases.
Need To Build Your Own Custom Cytometric Bead Array?
Choose from our list of over 200 validated antibody pairs. Our RayPlex development team can combine and optimize your chosen analytes into a precise and sensitive custom multiplex kit, empowering you with reliable, high-throughput results. Custom RayPlex bead array kits are typically ready to ship within 3 weeks.
