The Cytokine Array C5 is a membrane-based antibody array for screening and comparing expression levels of 80 human cytokines in any liquid sample type.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Number of Targets Detected | 80 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Species | Human |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Angiogenin
| BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| EGF
|
| ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| FGF-4
|
| FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
| GCP-2 (CXCL6)
| GCSF
| GDNF
| GM-CSF
| GRO alpha (CXCL1)
|
| GRO alpha/beta/gamma
| HGF
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
|
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-1 alpha (IL-1 F1)
|
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-5
|
| IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-12 p40/p70
|
| IL-13
| IL-15
| IL-16
| IP-10 (CXCL10)
| Leptin
|
| LIF
| LIGHT (TNFSF14)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
| MIF
| MIG (CXCL9)
|
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
| NT-4
| Oncostatin M
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
| PDGF-BB
| PLGF
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
| TARC (CCL17)
| TGF beta 1
| TGF beta 2
| TGF beta 3
| Thrombopoietin (TPO)
|
| TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| VEGF-A
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Celis J E, Gromov P, Cabezo T, Moreira JM Ambartsumian N, et al.Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment. Mol Cell Proteomics. 2004;3:327-344.
Species:
Human
Sample Type:
Interstitial Fluid (Tumor interstitial fluid)
Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1690
Species:
Human
Sample Type:
Conditioned Media (conditioned or non-conditioned medium)
Perotti V., Baldassari P., Bersani I., et al. NFATc2 is a potential therapeutic target in human melanoma. J Invest Dermatol. 2012 Nov;132(11):2652-60. doi: 10.1038/jid.2012.179
Species:
Human
Sample Type:
Conditioned Media (Melanoma Cell lines)
Canady, Johanna, et al. "Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis." Journal of Investigative Dermatology 133.3 (2013): 647-657.
Species:
Human
Sample Type:
Conditioned Media (keritinocytes stimulated with KGF)
Cherif H, Bisson DG, Mannarino M, Rabau O, Ouellet JA, Haglund L. Senotherapeutic drugs for human intervertebral disc degeneration and low back pain. Elife. 2020 Aug 21;9:e54693. doi: 10.7554/eLife.54693. PMID: 32821059; PMCID: PMC7442487.
Species:
Human
Sample Type:
Conditioned media (pellet cell culture)
Lietman C., Wu B., Lechner S., et al. Inhibition of Wnt/?-catenin signaling ameliorates osteoarthritis in a murine model of experimental osteoarthritis. JCI Insight. 2018 Feb 8;3(3). pii: 96308. doi: 10.1172/jci.insight.96308
Species:
Human
Sample Type:
Conditioned Media (Chondrocytes and synovial fluid from an osteoarthritis model)
Dominguez C., McCampbell K., David J., Palena C. Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer. JCI Insight. 2017 Nov 2;2(21). pii: 94296. doi: 10.1172/jci.insight.94296.
Species:
Human
Sample Type:
Conditioned Media (Breast cancer cell lines including MDB-MB-231 and MCF7 among others)
He T, Smith LA, Harrington S, Nath KA, Caplice NM, Katusic ZS. Transplantation of Circulating Endothelial Progenitor Cells Restores Endothelial Function of Denuded Rabbit Carotid Arteries. Stroke. 2004;35;2378-2384;
Species:
Rabbit
Sample Type:
Conditioned Media (endotheilial progentior cells (EPC) Cell-conditioned media)
Zhao D., Najbauer J., Annala AJ., et al. Human neural stem cell tropism to metastatic breast cancer. Stem Cells. 2012 Feb;30(2):314-25. doi: 10.1002/stem.784.
Species:
Human
Sample Type:
Conditioned Media (MDA-MB-231 breast cancer cells)
Huang L, Zhao A, Wong F, Ayala JM, et al. Leukotriene B4 Strongly Increases Monocyte Chemoattractant Protien-1 in Human Monocytes. Arterioscler Thromb Vasc Biol. 2004;24:1783-1788.
Species:
Human
Sample Type:
Conditioned Media (Monocytes)
- Great productI wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.
from University of Texas Health Science Center San Antonio,
on
- Great productI highly recommend this cytokine array kit! It is very easy to use and generates great results. Be sure to set up proper experimental conditions, I used cell culture supernates which yielded much less background than cell lysates.
from University of Texas Medical Branch Galveston,
on
- Great productWorks best for invitro mamalian cell cytokine assay
from Forsyth Institute,
on
- Great productI really liked this kit. It was very easy to use and came with detailed instructions as well troubleshooting tips. I have purchased another similar kit after using this. Highly recommend.
from UNC Chapel Hill,
on
- Great productThe protocol is easy to follow. This kit only needs a small sample volume and can detect a low concentration of cytokine signaling.
from University of Kansas,
on
- Great productThe Human Cytokine Array C5 kit gave me satisfied candidates under the conditioned medium. I strongly recommend this!!
from Winship Cancer Institute of Emory University,
on
- Great producteasy to use and analysis, got excellent results!
from NC State University,
on
- Great productSimple to use and detects many cytokines
from Johns Hopkins University School of Medicine,
on
- Great productIt works great using the cell growth media.
from Emory University,
on
- Great productI used on mammalian cell culture supernatant it and works well
from Indiana University,
on
- Great productKit worked well with conditioned media from smooth muscle cells. Provides a lot of data in a small amount of time.
from University of Nevada School of Medicine,
on
- Great productHuman Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.
on
- Great productEasy to use, works well.
from East Tennessee State University,
on
- Great productEasy to use, and protocols are easy to follow. Recommended to fellow colleagues.
from UCSF,
on
- Great productProtocol is simple and worked great with our conditioned media!
from UNMC,
on
- Great productSimple and easy protocol with clean results.
from Augusta University,
on
- Great productThe protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!
from Augusta University,
on
- Great productWorked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.
from Stanford University,
on
- Great productit's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.
from University of the pacific,
on
- Great productWorked great for med-high abundant proteins. Customer service was spectacular
from UCLA,
on
- Great productThis kit can detect huge amount of different cytokines and the price is only a little bit more than the kits that only test around 10 cytokines.
from Nanjing Medical University,
on
- Great productworks good with condition media
on
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