Human Cytokine Array C5 is a membrane-based multiplex antibody array for simultaneous screening of 80 cytokines with ELISA-level specificity and pg/ml sensitivity. Compatible with serum, plasma, lysates, and cell culture supernatants, it enables rapid cytokine profiling using a simple western blot-style workflow.
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 80 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
The Human Cytokine Array C5 is a multiplex, membrane-based antibody array designed for simultaneous screening of 80 human cytokines from a single sample. This high-density cytokine profiling platform combines sandwich ELISA specificity with the multiplex capability of protein arrays, enabling rapid comparison of cytokine expression patterns across experimental conditions.
Unlike traditional single-target ELISA assays or complex bead-based multiplex platforms, the Cytokine Array C5 provides a simple western blot-like workflow that can be completed in one day with minimal optimization. The array is compatible with serum, plasma, cell culture supernatants, tissue lysates, and cell lysates, making it ideal for biomarker discovery, inflammation research, and pathway analysis.
Learn More About Cytokine Arrays
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Angiogenin
|
BDNF
|
BLC
(CXCL13)
|
CK beta 8-1
(CCL23)
|
EGF
|
|
ENA-78
(CXCL5)
|
Eotaxin-1
(CCL11)
|
Eotaxin-2
(MPIF-2/CCL24)
|
Eotaxin-3
(CCL26)
|
FGF-4
|
|
FGF-6
|
FGF-7
(KGF)
|
FGF-9
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1)
|
|
GCP-2
(CXCL6)
|
GCSF
|
GDNF
|
GM-CSF
|
GRO alpha
(CXCL1)
|
|
GRO alpha/beta/gamma
|
HGF
|
I-309
(TCA-3/CCL1)
|
IFN-gamma
|
IGF-1
|
|
IGFBP-1
|
IGFBP-2
|
IGFBP-3
|
IGFBP-4
|
IL-1 alpha
(IL-1 F1)
|
|
IL-1 beta
(IL-1 F2)
|
IL-2
|
IL-3
|
IL-4
|
IL-5
|
|
IL-6
|
IL-7
|
IL-8
(CXCL8)
|
IL-10
|
IL-12 p40/p70
|
|
IL-13
|
IL-15
|
IL-16
|
IP-10
(CXCL10)
|
Leptin
|
|
LIF
|
LIGHT
(TNFSF14)
|
MCP-1
(CCL2)
|
MCP-2
(CCL8)
|
MCP-3
(MARC/CCL7)
|
|
MCP-4
(CCL13)
|
M-CSF
|
MDC
(CCL22)
|
MIF
|
MIG
(CXCL9)
|
|
MIP-1 beta
(CCL4)
|
MIP-1 delta
(CCL15)
|
MIP-3 alpha
(CCL20)
|
NAP-2
(PPBP/CXCL7)
|
NT-3
|
|
NT-4
|
Oncostatin M
|
Osteopontin
(SPP1)
|
Osteoprotegerin
(TNFRSF11B)
|
PARC
(CCL18)
|
|
PDGF-BB
|
PLGF
|
RANTES
(CCL5)
|
SCF
|
SDF-1 alpha
(CXCL12 alpha)
|
|
TARC
(CCL17)
|
TGF beta 1
|
TGF beta 2
|
TGF beta 3
|
Thrombopoietin
(TPO)
|
|
TIMP-1
|
TIMP-2
|
TNF alpha
|
TNF beta
(TNFSF1B)
|
VEGF-A
|
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Human Cytokine Array C5 is a membrane-based multiplex antibody array for simultaneous screening of 80 cytokines with ELISA-level specificity and pg/ml sensitivity. Compatible with serum, plasma, lysates, and cell culture supernatants, it enables rapid cytokine profiling using a simple western blot-style workflow.
Cytokine Array C5 Membrane Signals
Cytokine Array C5 Membrane Signals
Works well with TNBC cell sups and easy to follow protocol.
Review by Pari, from University of Florida, on Feb 13, 2026
Worked well with our media from THP-1 macrophage culture. Allows detection of a large amount of cytokines via an easy-to-follow protocol, it was a good introduction to antibody arrays for undergraduate students.
Review by Brakstin Hockley, from Ohio University, on May 27, 2025
I have been using these arrays for more than 7 years and they are great if you do not want to go on a big fishing expedition with things like RNA seq. The arrays help me focus on what I am looking for and save a lot of time and effort. The work flow also is very easy to follow and you get results in a day or two. Also I like that they are consistent and no variation from on batch to another .
Review by Someone, from Kansas City University, on Apr 16, 2024
The kit is easy to use. The results clearly showed the difference between treated and untreated media from human PBMCs. Excel sheets are also useful for quantifying results.
Review by S, jun, from duke university, on Mar 12, 2024
Works well with conditioned media from breast cancer cells
Review by Sahana, from University of Oklahoma Health Sciences center, on Feb 19, 2024
Great product. The protocol is easy to follow.
Review by Andrew Evans, from University of Kansas, on Dec 6, 2023
Worked great with condition media from non-tumorigenic cell lines.
Review by Luis Arteaga, from The University of kansas, on Dec 6, 2023
I highly recommend this cytokine array kit! It is very easy to use and generates great results. Be sure to set up proper experimental conditions, I used cell culture supernates which yielded much less background than cell lysates.
Review by Regina, from University of Texas Medical Branch Galveston, on Nov 3, 2022
Works best for invitro mamalian cell cytokine assay
Review by Deepak Chouhan, from Forsyth Institute, on Aug 8, 2022
I really liked this kit. It was very easy to use and came with detailed instructions as well troubleshooting tips. I have purchased another similar kit after using this. Highly recommend.
Review by Aditi Kothari, from UNC Chapel Hill, on Jul 11, 2022
The protocol is easy to follow. This kit only needs a small sample volume and can detect a low concentration of cytokine signaling.
Review by Zhang, from University of Kansas, on Apr 16, 2022
The Human Cytokine Array C5 kit gave me satisfied candidates under the conditioned medium. I strongly recommend this!!
Review by Jung, from Winship Cancer Institute of Emory University, on Jun 19, 2021
easy to use and analysis, got excellent results!
Review by Kenneth, from NC State University, on Apr 6, 2021
Simple to use and detects many cytokines
Review by Jose, from Johns Hopkins University School of Medicine, on Mar 18, 2021
It works great using the cell growth media.
Review by DYan, from Emory University, on Jan 20, 2021
I used on mammalian cell culture supernatant it and works well
Review by Vaishnavi, from Indiana University, on Jan 6, 2021
Kit worked well with conditioned media from smooth muscle cells. Provides a lot of data in a small amount of time.
Review by Craig, from University of Nevada School of Medicine, on Mar 20, 2020
I wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.
Review by Mubeen, from University of Texas Health Science Center San Antonio, on Mar 1, 2020
Easy to use, works well.
Review by Erick, from East Tennessee State University, on Jan 16, 2020
Easy to use, and protocols are easy to follow. Recommended to fellow colleagues.
Review by Raheel, from UCSF, on Dec 2, 2019
Protocol is simple and worked great with our conditioned media!
Review by S Chaudhary, from UNMC, on Jul 18, 2019
Simple and easy protocol with clean results.
Review by Manali, from Augusta University, on May 2, 2019
The protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!
Review by Thaiz, from Augusta University, on Dec 10, 2018
Worked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.
Review by Suchitra, from Stanford University, on Nov 12, 2018
it's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.
Review by Harmony, from University of the pacific, on Sep 12, 2018
Worked great for med-high abundant proteins. Customer service was spectacular
Review by P V raj, from UCLA, on Jul 19, 2018
This kit can detect huge amount of different cytokines and the price is only a little bit more than the kits that only test around 10 cytokines.
Review by Joe wong, from Nanjing Medical University, on May 8, 2018
works good with condition media
Review by Himanshu Arora, on Apr 25, 2018
Human Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.
Review by Kenneth, on Apr 10, 2018
