Recombinant Human RAD51 protein derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q06609 |
| Gene Id | 5888 |
| Gene Symbols | RAD51, RAD51A, RECA |
| Protein Name / Synonyms | DNA repair protein RAD51 homolog 1 (HsRAD51) (hRAD51) (RAD51 homolog A), RAD51 Recombinase, RAD51A, RECA, RAD51 Homolog A, HsRad51, HRAD51, MRMV2, RAD51 Homolog (RecA Homolog, E. Coli) (S. Cerevisiae), RAD51 (S. Cerevisiae) Homolog (E Coli RecA Homolog), BRCA1/BRCA2-Containing Complex, Subunit 5, DNA Repair Protein RAD51 Homolog 1, BRCA1/BRCA2-Containing Complex, RAD51 Homolog (S. Cerevisiae), RecA, E. Coli, Homolog Of, Recombination Protein A, RecA-Like Protein, Subunit 5, HsT16930, BRCC5. |
| Expressed Region | Ala2-Asp339 |
| Expression System | E. coli |
| Tag | N-terminal histidine tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 40 kDa |
| Format | Liquid |
| Formulation | Supplied as a 0.45 µm filtered solution in 50 mM Na2HPO4 (pH 7.4), 0.5 M NaCl, 440 mM imidazole, 6 M urea. |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bio-Rad protein assay dye reagent concentrate. |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cancer, DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
RAD51 Recombinase is a 37-kilodalton protein primarily expressed in proliferating cells and tissues undergoing DNA replication and repair. It is a key player in homologous recombination, a fundamental mechanism for repairing double-stranded DNA breaks and maintaining genomic stability. RAD51 forms nucleoprotein filaments on single-stranded DNA and facilitates strand exchange during the repair process. Dysregulation or mutations in the RAD51 gene are associated with genomic instability and an increased risk of cancer development.
Reference: https://pubmed.ncbi.nlm.nih.gov/15279796/
SDS-PAGE Image
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Disclaimer
"Antigen Grade" is not a formally regulated industry standard, but it is commonly used to indicate that a protein is intended for antigen-based applications rather than functional assays.
Antigen Grade proteins are recombinant proteins purified under denaturing conditions (e.g., in urea) and may not retain their native conformation or biological activity. These proteins are suitable for antigen-based applications such as antibody generation, ELISA plate coating, Western blotting, and other immunological assays, but are not recommended for functional or activity assays.
Suitable Applications:
✔ Antibody production (immunogen)✔ ELISA coating antigen
✔ Western blot positive control
✔ Dot blot
✔ Epitope mapping
✔ Antibody screening, etc.
Not Recommended:
✘ Enzyme activity assays✘ Ligand-receptor binding assays (unless validated)
✘ Cell-based functional assays
✘ SPR/BLI kinetic studies requiring native folding
Why "Antigen Grade"?
Many bacterial proteins expressed as inclusion bodies are purified in 6–8 M urea. Although they can often be refolded, proteins sold in urea are generally not guaranteed to be correctly refolded or biologically active. "Antigen Grade" proteins are suitable for immunological applications but may not be appropriate for assays requiring native protein function.