Rabbit Anti-AKT2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P31751 |
| Gene Id | 208 |
| Gene Symbols | AKT2 |
| Protein Name / Synonyms | AKT2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human AKT2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cancer, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Specificity
Other Names
AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta
NCBI Accession #
NP_001617.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55769
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat# 102-17082) in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab. |
 | Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat# 102-17082) in 293 cell line lysates transiently transfected with the AKT2 gene (2ug/lane). AKT2(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term)(Cat# 102-17082), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of AKT2 Antibody (N-term)(Cat# 102-17082) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | AKT2 Antibody (N-term) (Cat# 102-17082) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
