25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

The RayBio^® Alpha-B crystallin (CRYAB) Human IgM ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM autoantibody against CRYAB in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	6-8 weeks
Species	Human
Compatible Sample Types	Plasma, Serum
Accession Number	P02511
Gene Id	1410
Gene Symbols	CRYAB CRYA2 HSPB5
Protein Name / Synonyms	Alpha-crystallin B chain (Alpha(B)-crystallin) (Heat shock protein beta-5) (HspB5) (Renal carcinoma antigen NY-REN-27) (Rosenthal fiber component)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Indirect
Method Of Detection	Colorimetric
Research Area	Cardiovascular Disease, Autoimmune Disease
Shipping Type	Dry ice (additional shipping fee)
Storage	-20°C

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Detection Antibody
Biotinylated Anti-Positive Control
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
Sample Diluent

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 µL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (IEQ-PRELIM.pdf)

SDS (IEQ-PRELIM.pdf)

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Detection Antibody
Biotinylated Anti-Positive Control
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
Sample Diluent

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 µL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Featured Products

25% off all ELISA kits

25% off all ELISA kits

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Alpha-B crystallin (CRYAB) Human IgM ELISA KitAlpha-B crystallin (CRYAB) Human IgM ELISA Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

25% off all ELISA kits

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

Featured Products

25% off all ELISA kits

25% off all ELISA kits

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Alpha-B crystallin (CRYAB) Human IgM ELISA KitAlpha-B crystallin (CRYAB) Human IgM ELISA Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

25% off all ELISA kits

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit

Alpha-B crystallin (CRYAB) Human IgM ELISA Kit