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Anti-3 X Flag Antibody
Catalog #: 130-10648
Mouse Anti-Human 3 X Flag (3D12-H10-F8) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone | 3D12-H10-F8 |
| Preparation | Immunogen was polypeptidet of 3 X Flag. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
3 X Flag peptide has found widespread use as a mild purification reagent for Flag-epitope tagged recombinant proteins. Ability to bind heavy metals was a significant finding. 3 X FLAG epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. FLAG-tag can be removed by treatment with enterokinase, which is specific for the five C-terminal amino acids of the peptide sequence5.
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.