Anti-A2M Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P01023
Gene Id	2
Gene Symbols	A2M
Protein Name / Synonyms	A2M, A2MD, CPAMD5, FWP007, S863-7, alpha-2-macroglobulin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

163kDa

Observed MW

180kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1155-1474 of human A2M (NP_000005.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using A2M antibody (144-01573) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - A2M Polyclonal Antibody (144-01573)

Immunofluorescence analysis of 293T cells using A2M antibody (144-01573) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - A2M Polyclonal Antibody (144-01573)

Expiration:

12 months from the date of shipment when stored properly.

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