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Anti-AAAS Antibody
Catalog #: 144-06427
AAAS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9NRG9 |
| Gene Id | 8086 |
| Gene Symbols | AAAS |
| Protein Name / Synonyms | AAAS, AAA, AAASb, ADRACALA, ADRACALIN, ALADIN, GL003, aladin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
55kDa/59kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 287-546 of human AAAS (NP_056480.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AAAS antibody (144-06427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (144-06427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AAAS Antibody (144-06427) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using AAAS Antibody (144-06427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (144-06427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AAAS Antibody (144-06427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AAAS Antibody (144-06427) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AAAS antibody (144-06427). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.