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Anti-AARS2 Antibody

Catalog #: 144-07826

AARS2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q5JTZ9
Gene Id
57505
Gene Symbols
AARS2
Protein Name / Synonyms
AARS2, AARSL, COXPD8, LKENP, MT-ALARS, MTALARS, alanine--tRNA ligase, mitochondrial
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
107kDa
Observed MW
107kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 841-985 of human AARS2 (NP_065796.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AARS2 antibody (144-07826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - AARS2 antibody (144-07826)

Immunohistochemistry of paraffin-embedded human liver injury using AARS2 antibody (144-07826) at dilution of 1:100 (40x lens).
Immunohistochemistry - AARS2 antibody (144-07826)

Expiration:
12 months from the date of shipment when stored properly.