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Anti-ABCB8 Antibody
Catalog #: 144-02653
ABCB8 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NUT2 |
| Gene Id | 11194 |
| Gene Symbols | ABCB8 |
| Protein Name / Synonyms | ABCB8, EST328128, M-ABC1, MABC1, ATP binding cassette subfamily B member 8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
59kDa/68kDa/75kDa/78kDa/79kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ABCB8 antibody (144-02653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver injury using ABCB8 antibody (144-02653) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (144-02653) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (144-02653) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.