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Anti-ABL1 Antibody
Catalog #: 144-00282
ABL1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P00519 |
| Gene Id | 25 |
| Gene Symbols | ABL1 |
| Protein Name / Synonyms | ABL1, ABL, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl, tyrosine-protein kinase ABL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
122kDa/124kDa
Observed MW
139kDa
Immunogen
A synthetic peptide of human ABL1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using ABL1 antibody (144-00282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using ABL1 antibody (144-00282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ABL1 antibody (144-00282) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cells using ABL1 antibody (144-00282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.