Anti-ACADM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P11310
Gene Id	34
Gene Symbols	ACADM
Protein Name / Synonyms	ACADM, ACAD1, MCAD, MCADH, acyl-CoA dehydrogenase, C-4 to C-12 straight chain
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

46kDa/47kDa

Observed MW

46kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ACADM antibody (144-01873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - ACADM Polyclonal Antibody (144-01873)

Immunofluorescence analysis of HeLa cells using ACADM antibody (144-01873) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - ACADM Polyclonal Antibody (144-01873)

Expiration:

12 months from the date of shipment when stored properly.

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