Anti-ACADS Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P16219
Gene Id	35
Gene Symbols	ACADS
Protein Name / Synonyms	ACADS, ACAD3, SCAD, acyl-CoA dehydrogenase, C-2 to C-3 short chain
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

44kDa

Observed MW

44kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ACADS antibody (144-07230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - ACADS Polyclonal Antibody (144-07230)

Immunofluorescence analysis of MCF7 cells using ACADS antibody (144-07230). Blue: DAPI for nuclear staining.
Immunofluorescence - ACADS Polyclonal Antibody (144-07230)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ACADS antibody (144-07230). Western blot was performed from the immunoprecipitate using ACADS antibody (144-07230) at a dilition of 1:1000.
Immunoprecipitation - ACADS Polyclonal Antibody (144-07230)

Expiration:

12 months from the date of shipment when stored properly.

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