Anti-ACE2 (SARS Receptor) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9BYF1
Gene Id	59272
Gene Symbols	ACE2
Protein Name / Synonyms	ACE2 (SARS Receptor) (Center)
Clonality	Polyclonal
Preparation	This ACE2 (SARS Receptor) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-172 amino acids from the Central region of human ACE2 (SARS Receptor).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.

Specificity

Other Names

ACE2; Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; Processed angiotensin-converting enzyme 2

NCBI Accession #

NP_068576.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92463

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from human kidney tissue lysate, using ACE2(SARS-R) Antibody (S155)(Cat# 102-16640). 102-16640 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Flow cytometric analysis of HL-60 cells using ACE2 (SARS Receptor) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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