Anti-ACO2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q99798
Gene Id	50
Gene Symbols	ACO2
Protein Name / Synonyms	ACO2, ACONM, HEL-S-284, ICRD, OCA8, OPA9, aconitase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

85kDa

Observed MW

105kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human ACO2 (NP_001089.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ACO2 antibody (144-03716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - ACO2 Polyclonal Antibody (144-03716)

Immunofluorescence analysis of HeLa cells using ACO2 antibody (144-03716) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - ACO2 Polyclonal Antibody (144-03716)

Expiration:

12 months from the date of shipment when stored properly.

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