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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15067 |
| Gene Id | 51 |
| Gene Symbols | ACOX1 |
| Protein Name / Synonyms | ACOX1, ACOX, PALMCOX, SCOX, acyl-CoA oxidase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
70kDa/74kDa
Observed MW
74kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACOX1 (NP_004026.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ACOX1 antibody (144-08091) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using ACOX1 antibody (144-08091) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ACOX1 antibody (144-08091) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.