RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-ACP5 Antibody

Catalog #: 144-02528

ACP5 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P13686
Gene Id
54
Gene Symbols
ACP5
Protein Name / Synonyms
ACP5, HPAP, TRACP5a, TRACP5b, TRAP, TrATPase, acid phosphatase 5, tartrate resistant
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer, Inflammation, Autoimmune Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
36kDa
Observed MW
37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 76-325 of human ACP5 (NP_001602.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ACP5 antibody (144-02528) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - ACP5 antibody (144-02528)

Immunofluorescence analysis of U2OS cells using ACP5 antibody (144-02528). Blue: DAPI for nuclear staining.
Immunofluorescence - ACP5 antibody (144-02528)

Expiration:
12 months from the date of shipment when stored properly.