Anti-ACTA2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P62736
Gene Id	59
Gene Symbols	ACTA2
Protein Name / Synonyms	ACTA2, AAT6, ACTSA, MYMY5, actin, aortic smooth muscle
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Dilutions

WB 1:500 - 1:1000
IHC 1:50 - 1:100

Format

Calculated MW

42kDa

Observed MW

42kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human ACTA2 (NP_001135417.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ACTA2 antibody (144-60050) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - ACTA2 Polyclonal Antibody (144-60050)

Immunohistochemistry of paraffin-embedded human oophoroma using ACTA2 antibody (144-60050) at dilution of 1:100 (40x lens).
Immunohistochemistry - ACTA2 Polyclonal Antibody (144-60050)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products