3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-ACTR2 Antibody
Catalog #: 144-05734
ACTR2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P61160 |
| Gene Id | 10097 |
| Gene Symbols | ACTR2 |
| Protein Name / Synonyms | ACTR2, ARP2, actin-related protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
44kDa/45kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2 (NP_005713.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ACTR2 antibody (144-05734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot analysis of extracts of various cells, using ACTR2 antibody (144-05734) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody (144-05734) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.