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Anti-ACVR2A Antibody
Catalog #: 144-01981
ACVR2A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P27037 |
| Gene Id | 92 |
| Gene Symbols | ACVR2A |
| Protein Name / Synonyms | ACVR2A, ACTRII, ACVR2, activin receptor type-2A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
45kDa/57kDa
Observed MW
57kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human ACVR2A (NP_001607.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ACVR2A antibody (144-01981) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cells using ACVR2A antibody (144-01981) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.