RayBiotech

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ISO 13485:2016 cGMP

Anti-ADAM9 Antibody

Catalog #: 144-05388

ADAM9 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q13443
Gene Id
8754
Gene Symbols
ADAM9
Protein Name / Synonyms
ADAM9, CORD9, MCMP, MDC9, Mltng, ADAM metallopeptidase domain 9
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
72kDa/90kDa
Observed MW
100kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 475-685 of human ADAM9 (NP_003807.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ADAM9 antibody (144-05388) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ADAM9 antibody (144-05388)

Immunofluorescence analysis of U2OS cells using ADAM9 antibody (144-05388). Blue: DAPI for nuclear staining.
Immunofluorescence - ADAM9 antibody (144-05388)

Expiration:
12 months from the date of shipment when stored properly.