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Anti-ADAR Antibody
Catalog #: 144-07869
ADAR Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P55265 |
| Gene Id | 103 |
| Gene Symbols | ADAR |
| Protein Name / Synonyms | ADAR, ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, adenosine deaminase, RNA specific |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
103kDa/131kDa/133kDa/136kDa/140kDa
Observed MW
110kDa, 150kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ADAR antibody (144-07869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ADAR antibody (144-07869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.