Anti-ADH4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P08319
Gene Id	127
Gene Symbols	ADH4
Protein Name / Synonyms	ADH4, ADH-2, HEL-S-4, alcohol dehydrogenase 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

40kDa/42kDa

Observed MW

45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ADH4 (NP_000661.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse liver, using ADH4 antibody (144-05454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - ADH4 antibody (144-05454)

Immunohistochemistry of paraffin-embedded rat kidney using ADH4 antibody (144-05454) at dilution of 1:200 (40x lens).
Immunohistochemistry - ADH4 antibody (144-05454)

Expiration:

12 months from the date of shipment when stored properly.

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