Anti-ADRBK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P25098
Gene Id	156
Gene Symbols	ADRBK1
Protein Name / Synonyms	GRK2, ADRBK1, BARK1, BETA-ARK1, beta-adrenergic receptor kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

79kDa

Observed MW

80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (144-01662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Western blot analysis of extracts from normal (control) and ADRBK1 knockout (KO) 293T cells, using ADRBK1 antibody (144-01662) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Immunohistochemistry of paraffin-embedded rat heart using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)

Expiration:

12 months from the date of shipment when stored properly.

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