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Anti-ADSL Antibody
Catalog #: 144-06278
ADSL Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P30566 |
| Gene Id | 158 |
| Gene Symbols | ADSL |
| Protein Name / Synonyms | ADSL, AMPS, ASASE, ASL, adenylosuccinate lyase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the lyase 1 family. It is an essential enzyme involved in purine metabolism, and catalyzes two non-sequential reactions in the de novo purine biosynthetic pathway: the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR) and the conversion of adenylosuccinate (S-AMP) to adenosine monophosphate (AMP). Mutations in this gene are associated with adenylosuccinase deficiency (ADSLD), a disorder marked with psychomotor retardation, epilepsy or autistic features. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:20 - 1:100
IP 1:50 - 1:100
IF 1:20 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
48kDa/54kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ADSL (NP_000017.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ADSL antibody (144-06278) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using ADSL antibody (144-06278). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.