AFF4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UHB7 |
| Gene Id | 27125 |
| Gene Symbols | AFF4 |
| Protein Name / Synonyms | AFF4, AF5Q31, CHOPS, MCEF, AF4/FMR2 family member 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
38kDa/98kDa/127kDa
Observed MW
150kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AFF4 antibody (144-04644) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Expiration:
12 months from the date of shipment when stored properly.