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Anti-AGFG1 Antibody
Catalog #: 144-06294
AGFG1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P52594 |
| Gene Id | 3267 |
| Gene Symbols | AGFG1 |
| Protein Name / Synonyms | AGFG1, HRB, RAB, RIP, ArfGAP with FG repeats 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
54kDa/58kDa/60kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AGFG1 antibody (144-06294) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.