RayBiotech

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Anti-AGK Antibody

Catalog #: 144-09976

AGK Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Rat
Host SpeciesRabbit
Accession Number
Q53H12
Gene Id
55750
Gene Symbols
AGK
Protein Name / Synonyms
AGK, CATC5, CTRCT38, MTDPS10, MULK, acylglycerol kinase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
7kDa/47kDa
Observed MW
47kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK (NP_060708.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AGK antibody (144-09976) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - AGK antibody (144-09976)

Expiration:
12 months from the date of shipment when stored properly.