Anti-AGO2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UKV8
Gene Id	27161
Gene Symbols	AGO2
Protein Name / Synonyms	AGO2, CASC7, EIF2C2, LINC00980, PPD, Q10, protein argonaute-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

93kDa/97kDa

Observed MW

100kDa

Immunogen

Recombinant protein of human AGO2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AGO2 antibody (144-06802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - AGO2 antibody (144-06802)

Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Immunohistochemistry - AGO2 antibody (144-06802)

Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (144-06802) at dilution of 1:100 (40x lens).
Immunohistochemistry - AGO2 antibody (144-06802)