Anti-AGPAT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q99943
Gene Id	10554
Gene Symbols	AGPAT1
Protein Name / Synonyms	AGPAT1, 1-AGPAT1, G15, LPAAT-alpha, LPAATA, 1-acylglycerol-3-phosphate O-acyltransferase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW

31kDa

Observed MW

32kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (144-06517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - AGPAT1 Polyclonal Antibody (144-06517)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody (144-06517). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (144-06517) at a dilition of 1:1000.
Immunoprecipitation - AGPAT1 Polyclonal Antibody (144-06517)

Expiration:

12 months from the date of shipment when stored properly.

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