Anti-AGT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P01019
Gene Id	183
Gene Symbols	AGT
Protein Name / Synonyms	AGT, ANHU, SERPINA8, hFLT1, angiotensinogen
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

53kDa

Observed MW

53kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human AGT (NP_000020.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of HepG2 cells, using AGT antibody (144-015637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - AGT Polyclonal Antibody (144-015637)

Expiration:

12 months from the date of shipment when stored properly.

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