RayBiotech

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ISO 13485:2016 cGMP

Anti-AGXT Antibody

Catalog #: 144-08397

AGXT Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P21549
Gene Id
189
Gene Symbols
AGXT
Protein Name / Synonyms
AGXT, AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6, serine--pyruvate aminotransferase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
43kDa
Observed MW
43kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AGXT antibody (144-08397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - AGXT antibody (144-08397)

Expiration:
12 months from the date of shipment when stored properly.