AHCYL2 antibody [N3C3], 0.66 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 23382 |
| Protein Name / Synonyms | AHCYL2 |
| Concentration (lot specific) | 0.66 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ADOHCYASE3 antibody, FLJ21719 antibody, KIAA0828 antibody, AHCYL2 antibody, putative adenosylhomocysteinase 3 antibody, S-adenosyl-L-homocysteine hydrolase 3 antibody, S-adenosylhomocysteine hydrolase-like 2 antibody, adenosylhomocysteinase-like 2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
67 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 298 and 578 of AHCYL2 (Uniprot ID# Q96HN2)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
AHCYL2 antibody [N3C3] detects AHCYL2 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHCYL2 protein stained by AHCYL2 antibody [N3C3] (107-11011) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHCYL2 protein stained by AHCYL2 antibody [N3C3] (107-11011) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using AHCYL2(107-11011) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
107-11011 diluted at 1:1000
A: Raji
7.5% SDS PAGE
107-11011 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.